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Biophytis 20e bio101
<t>20-hydroxyecdysone</t> <t>(20E;</t> β-ecdysone; crustecdysone; ecdysterone; <t>BIO101;</t> CAS 5289-74-7; IUPAC 2β,3β,14α,20 R ,22 R ,25-hexahydroxy-5β-cholest-7-en-6-one).

<t>20-hydroxyecdysone</t> <t>(20E;</t> β-ecdysone; crustecdysone; ecdysterone; <t>BIO101;</t> CAS 5289-74-7; IUPAC 2β,3β,14α,20 R ,22 R ,25-hexahydroxy-5β-cholest-7-en-6-one).

20e Bio101, supplied by Biophytis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/20e bio101/product/Biophytis
Average 90 stars, based on 1 article reviews

20e bio101 - by Bioz Stars, 2026-05

90/100 stars

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1) Product Images from "20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases"

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

Journal: Biomedicines

doi: 10.3390/biomedicines9050492

20-hydroxyecdysone (20E; β-ecdysone; crustecdysone; ecdysterone; BIO101; CAS 5289-74-7; IUPAC 2β,3β,14α,20 R ,22 R ,25-hexahydroxy-5β-cholest-7-en-6-one).

Figure Legend Snippet: 20-hydroxyecdysone (20E; β-ecdysone; crustecdysone; ecdysterone; BIO101; CAS 5289-74-7; IUPAC 2β,3β,14α,20 R ,22 R ,25-hexahydroxy-5β-cholest-7-en-6-one).

Techniques Used:

Selected examples of ecdysteroid-rich medicinal plants and their traditional uses. The names of ecdysteroids are underlined (see www.ecdybase.org for structures, accessed on 1 April 2021). Note that it is not established that all the reported biological activities are due totally or partly to the presence of ecdysteroids.

Figure Legend Snippet: Selected examples of ecdysteroid-rich medicinal plants and their traditional uses. The names of ecdysteroids are underlined (see www.ecdybase.org for structures, accessed on 1 April 2021). Note that it is not established that all the reported biological activities are due totally or partly to the presence of ecdysteroids.

Techniques Used:

Figure Legend Snippet: Summary of the literature concerning 20E’s pharmacological effects in mammals.

Techniques Used:

$( A ) The effects of 20E (0.01–10 μM) on protein synthesis in C2C12 cells, showing the anabolic effect of 20E on optimal value for 1 µM 20E. C2C12 cells were grown on 24-well plates at a density of 30,000 cells/well in 0.5 mL of growth medium (Dulbecco’s Modified Eagle Medium (DMEM) 4.5 g/L glucose supplemented with 10% fetal bovine serum). Twenty-four hours after plating, the differentiation induction into multinucleated myotubes was carried out, and after 5 days, cells were pre-incubated in Krebs medium 1 h at 37 °C before being incubated in DMEM media without serum for 2.5 h in the presence of [ 3 H]-Leucine (5 µCi/mL) and DMSO (control condition) or IGF-1 (100 ng/mL) or 20E (0.01–0.1–1–10 µM). At the end of incubation, supernatants were discarded and cells were lysed in 0.1 N NaOH for 30 min. The cell soluble fraction-associated radioactivity was then counted and protein was quantified using the Lowry method (after ). ( B ) Effects of dexamethasone (Dexa 6 = 10 −6 M, Dexa 5 = 10 −5 M), IGF-1 (10 ng/mL), and 20E (10 −6 M) on the diameter of C2C12 myotubes. Four- to six-day-old myotubes were incubated for 48 h with test chemicals, and were fixed and photographed by glutaraldehyde-induced autofluorescence. *: p = < 0.05; **: p = 0.01. (redrawn and modified from ).$
Figure Legend Snippet: ( A ) The effects of 20E (0.01–10 μM) on protein synthesis in C2C12 cells, showing the anabolic effect of 20E on optimal value for 1 µM 20E. C2C12 cells were grown on 24-well plates at a density of 30,000 cells/well in 0.5 mL of growth medium (Dulbecco’s Modified Eagle Medium (DMEM) 4.5 g/L glucose supplemented with 10% fetal bovine serum). Twenty-four hours after plating, the differentiation induction into multinucleated myotubes was carried out, and after 5 days, cells were pre-incubated in Krebs medium 1 h at 37 °C before being incubated in DMEM media without serum for 2.5 h in the presence of [ 3 H]-Leucine (5 µCi/mL) and DMSO (control condition) or IGF-1 (100 ng/mL) or 20E (0.01–0.1–1–10 µM). At the end of incubation, supernatants were discarded and cells were lysed in 0.1 N NaOH for 30 min. The cell soluble fraction-associated radioactivity was then counted and protein was quantified using the Lowry method (after ). ( B ) Effects of dexamethasone (Dexa 6 = 10 −6 M, Dexa 5 = 10 −5 M), IGF-1 (10 ng/mL), and 20E (10 −6 M) on the diameter of C2C12 myotubes. Four- to six-day-old myotubes were incubated for 48 h with test chemicals, and were fixed and photographed by glutaraldehyde-induced autofluorescence. *: p = < 0.05; **: p = 0.01. (redrawn and modified from ).

Techniques Used: Modification, Incubation, Control, Radioactivity

Dose-dependent inhibition of myostatin gene expression in C2C12 cells by 20E. C2C12 mouse myoblasts were differentiated for 6 days into myotubes. They were then treated for 6 h with concentrations of 20E ranging from 0.001 to 10 μM. Myostatin gene expression was detected by qRT-PCR. Results are shown as means ± standard error of the mean (SEM) ().

Figure Legend Snippet: Dose-dependent inhibition of myostatin gene expression in C2C12 cells by 20E. C2C12 mouse myoblasts were differentiated for 6 days into myotubes. They were then treated for 6 h with concentrations of 20E ranging from 0.001 to 10 μM. Myostatin gene expression was detected by qRT-PCR. Results are shown as means ± standard error of the mean (SEM) ().

Techniques Used: Inhibition, Gene Expression, Quantitative RT-PCR

Effect of 20E on mice fed a high-fat diet (HF), when compared to mice fed a low-fat diet (LF). The animals received either pure 20E (50 mg/kg/day) or the same amount of 20E as a quinoa extract (Q). Panel ( A ) shows the impact on the mass of epididymal adipose tissue (*** p < 0.01 when compared to LF; ## p < 0.01 and ### p < 0.001 when compared to HF) and panel ( B ) shows the effect on adipocyte diameter (reproduced, with permission, from ).

Figure Legend Snippet: Effect of 20E on mice fed a high-fat diet (HF), when compared to mice fed a low-fat diet (LF). The animals received either pure 20E (50 mg/kg/day) or the same amount of 20E as a quinoa extract (Q). Panel ( A ) shows the impact on the mass of epididymal adipose tissue (*** p < 0.01 when compared to LF; ## p < 0.01 and ### p < 0.001 when compared to HF) and panel ( B ) shows the effect on adipocyte diameter (reproduced, with permission, from ).

Techniques Used:

20E reduction of myostatin gene expression in C2C12 cells (differentiated for 6 days into myotubules) is mediated by binding to receptor sites on the external surface of the cells. The histogram compares the activities of IGF-1 (100 nM) and 20E (10 μM) with those of conjugates of 20E covalently bound to protein (HSA or BSA) through different C-atoms (C-2 and C-22-hemisuccinates or C-6 [6-carboxymethoxime]), all at nominal 10 μM hapten concentration. BSA bovine serum albumin; HSA: human serum albumin; error bars = standard error of the mean [ , ].

Figure Legend Snippet: 20E reduction of myostatin gene expression in C2C12 cells (differentiated for 6 days into myotubules) is mediated by binding to receptor sites on the external surface of the cells. The histogram compares the activities of IGF-1 (100 nM) and 20E (10 μM) with those of conjugates of 20E covalently bound to protein (HSA or BSA) through different C-atoms (C-2 and C-22-hemisuccinates or C-6 [6-carboxymethoxime]), all at nominal 10 μM hapten concentration. BSA bovine serum albumin; HSA: human serum albumin; error bars = standard error of the mean [ , ].

Techniques Used: Gene Expression, Binding Assay, Concentration Assay

Diagrammatic representation of the proposed mode of action of 20E in the regulation of protein synthesis in C2C12 muscle cells in vitro ().

Figure Legend Snippet: Diagrammatic representation of the proposed mode of action of 20E in the regulation of protein synthesis in C2C12 muscle cells in vitro ().

Techniques Used: In Vitro

Time-course of the distribution of radioactivity in the stomach/intestine/feces in mice after the oral application of [1α,2α- 3 H 2 ]20E. Note the logarithmic scale for abscissa. Each value is a mean of 2 animals. Note the plateau of small intestine content that is best explained by an entero-hepatic cycle and consistent with the prolonged concentration of radioactivity in bile as observed by Wu et al. (reproduced, with permission, from ).

Figure Legend Snippet: Time-course of the distribution of radioactivity in the stomach/intestine/feces in mice after the oral application of [1α,2α- 3 H 2 ]20E. Note the logarithmic scale for abscissa. Each value is a mean of 2 animals. Note the plateau of small intestine content that is best explained by an entero-hepatic cycle and consistent with the prolonged concentration of radioactivity in bile as observed by Wu et al. (reproduced, with permission, from ).

Techniques Used: Radioactivity, Concentration Assay

Comparison of the temporal ( A ) and cumulative ( B ) urinary and fecal elimination of radioactivity after the oral application of [5,7,9- 3 H]20E to 6–7 week-old male Wistar rats. After oral application of [ 3 H]20E, elimination of radioactively labelled 20E and its metabolites is completed within 48 h. The amount of radioactivity recovered from the feces is by far the major route of excretion, since the amount in the urine corresponds to only 1.40% of the total radioactivity recovered. (Reproduced, with permission, from ).

Figure Legend Snippet: Comparison of the temporal ( A ) and cumulative ( B ) urinary and fecal elimination of radioactivity after the oral application of [5,7,9- 3 H]20E to 6–7 week-old male Wistar rats. After oral application of [ 3 H]20E, elimination of radioactively labelled 20E and its metabolites is completed within 48 h. The amount of radioactivity recovered from the feces is by far the major route of excretion, since the amount in the urine corresponds to only 1.40% of the total radioactivity recovered. (Reproduced, with permission, from ).

Techniques Used: Comparison, Radioactivity

Kinetic parameters of BIO101 (=97% pure 20E) after a single oral administration ( n = 6, fasted). Values in parentheses indicate the SEM (C max , AUC) or the range (t max ).

Figure Legend Snippet: Kinetic parameters of BIO101 (=97% pure 20E) after a single oral administration ( n = 6, fasted). Values in parentheses indicate the SEM (C max , AUC) or the range (t max ).

Techniques Used:

Figure Legend Snippet: Pharmacokinetics of 20E in young and elderly humans given a single dose of 1400 mg. 20E was quantified by HPLC-MS [ , ].

Techniques Used: Drug discovery

The principal routes of metabolism of 20E in rodents and humans (modified from Kumpun et al., 2011). The circles and arrows in red highlight the changes associated with 14-dehydroxylation, while the boxes and arrows in blue highlight the changes associated with side-chain cleavage.

Figure Legend Snippet: The principal routes of metabolism of 20E in rodents and humans (modified from Kumpun et al., 2011). The circles and arrows in red highlight the changes associated with 14-dehydroxylation, while the boxes and arrows in blue highlight the changes associated with side-chain cleavage.

Techniques Used: Modification

Figure Legend Snippet: A representative flow-diagram for the large-scale extraction and purification of 20E from roots of Cyanotis sp.

Techniques Used: Extraction, Purification

Figure Legend Snippet: Drugability scores for ecdysteroids.

Techniques Used:

Figure Legend Snippet: Clinical studies on humans using pure 20E.

Techniques Used: Activity Assay, Clinical Proteomics, Control

SARS-CoV-2 infection will result in a strong impairment of the activity of angiotensin converting enzyme 2 (ACE2), hence a lack of angiotensin-(1-7) production and a disequilibrium between the harmful and protective arms of the renin–angiotensin system (RAS). Treatment with BIO101 (20E) is expected to activate Mas receptor and the protective arm of RAS, thus preventing inflammation and lung damage.

Figure Legend Snippet: SARS-CoV-2 infection will result in a strong impairment of the activity of angiotensin converting enzyme 2 (ACE2), hence a lack of angiotensin-(1-7) production and a disequilibrium between the harmful and protective arms of the renin–angiotensin system (RAS). Treatment with BIO101 (20E) is expected to activate Mas receptor and the protective arm of RAS, thus preventing inflammation and lung damage.

Techniques Used: Infection, Activity Assay

Image Search Results

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: 20-hydroxyecdysone (20E; β-ecdysone; crustecdysone; ecdysterone; BIO101; CAS 5289-74-7; IUPAC 2β,3β,14α,20 R ,22 R ,25-hexahydroxy-5β-cholest-7-en-6-one).

Article Snippet: It is proposed that RAS balance could be restored in COVID-19 patients through Mas1 activation downstream of ACE2 activity, with 20E (BIO101), a non-peptidic Mas receptor (Mas1) activator developed by Biophytis ( ).

Techniques:

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Selected examples of ecdysteroid-rich medicinal plants and their traditional uses. The names of ecdysteroids are underlined (see www.ecdybase.org for structures, accessed on 1 April 2021). Note that it is not established that all the reported biological activities are due totally or partly to the presence of ecdysteroids.

Techniques:

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Summary of the literature concerning 20E’s pharmacological effects in mammals.

Techniques:

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: ( A ) The effects of 20E (0.01–10 μM) on protein synthesis in C2C12 cells, showing the anabolic effect of 20E on optimal value for 1 µM 20E. C2C12 cells were grown on 24-well plates at a density of 30,000 cells/well in 0.5 mL of growth medium (Dulbecco’s Modified Eagle Medium (DMEM) 4.5 g/L glucose supplemented with 10% fetal bovine serum). Twenty-four hours after plating, the differentiation induction into multinucleated myotubes was carried out, and after 5 days, cells were pre-incubated in Krebs medium 1 h at 37 °C before being incubated in DMEM media without serum for 2.5 h in the presence of [ 3 H]-Leucine (5 µCi/mL) and DMSO (control condition) or IGF-1 (100 ng/mL) or 20E (0.01–0.1–1–10 µM). At the end of incubation, supernatants were discarded and cells were lysed in 0.1 N NaOH for 30 min. The cell soluble fraction-associated radioactivity was then counted and protein was quantified using the Lowry method (after ). ( B ) Effects of dexamethasone (Dexa 6 = 10 −6 M, Dexa 5 = 10 −5 M), IGF-1 (10 ng/mL), and 20E (10 −6 M) on the diameter of C2C12 myotubes. Four- to six-day-old myotubes were incubated for 48 h with test chemicals, and were fixed and photographed by glutaraldehyde-induced autofluorescence. *: p = < 0.05; **: p = 0.01. (redrawn and modified from ).

Techniques: Modification, Incubation, Control, Radioactivity

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Dose-dependent inhibition of myostatin gene expression in C2C12 cells by 20E. C2C12 mouse myoblasts were differentiated for 6 days into myotubes. They were then treated for 6 h with concentrations of 20E ranging from 0.001 to 10 μM. Myostatin gene expression was detected by qRT-PCR. Results are shown as means ± standard error of the mean (SEM) ().

Techniques: Inhibition, Gene Expression, Quantitative RT-PCR

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Effect of 20E on mice fed a high-fat diet (HF), when compared to mice fed a low-fat diet (LF). The animals received either pure 20E (50 mg/kg/day) or the same amount of 20E as a quinoa extract (Q). Panel ( A ) shows the impact on the mass of epididymal adipose tissue (*** p < 0.01 when compared to LF; ## p < 0.01 and ### p < 0.001 when compared to HF) and panel ( B ) shows the effect on adipocyte diameter (reproduced, with permission, from ).

Techniques:

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: 20E reduction of myostatin gene expression in C2C12 cells (differentiated for 6 days into myotubules) is mediated by binding to receptor sites on the external surface of the cells. The histogram compares the activities of IGF-1 (100 nM) and 20E (10 μM) with those of conjugates of 20E covalently bound to protein (HSA or BSA) through different C-atoms (C-2 and C-22-hemisuccinates or C-6 [6-carboxymethoxime]), all at nominal 10 μM hapten concentration. BSA bovine serum albumin; HSA: human serum albumin; error bars = standard error of the mean [ , ].

Techniques: Gene Expression, Binding Assay, Concentration Assay

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Diagrammatic representation of the proposed mode of action of 20E in the regulation of protein synthesis in C2C12 muscle cells in vitro ().

Techniques: In Vitro

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Time-course of the distribution of radioactivity in the stomach/intestine/feces in mice after the oral application of [1α,2α- 3 H 2 ]20E. Note the logarithmic scale for abscissa. Each value is a mean of 2 animals. Note the plateau of small intestine content that is best explained by an entero-hepatic cycle and consistent with the prolonged concentration of radioactivity in bile as observed by Wu et al. (reproduced, with permission, from ).

Techniques: Radioactivity, Concentration Assay

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Comparison of the temporal ( A ) and cumulative ( B ) urinary and fecal elimination of radioactivity after the oral application of [5,7,9- 3 H]20E to 6–7 week-old male Wistar rats. After oral application of [ 3 H]20E, elimination of radioactively labelled 20E and its metabolites is completed within 48 h. The amount of radioactivity recovered from the feces is by far the major route of excretion, since the amount in the urine corresponds to only 1.40% of the total radioactivity recovered. (Reproduced, with permission, from ).

Techniques: Comparison, Radioactivity

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Kinetic parameters of BIO101 (=97% pure 20E) after a single oral administration ( n = 6, fasted). Values in parentheses indicate the SEM (C max , AUC) or the range (t max ).

Techniques:

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Pharmacokinetics of 20E in young and elderly humans given a single dose of 1400 mg. 20E was quantified by HPLC-MS [ , ].

Techniques: Drug discovery

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: The principal routes of metabolism of 20E in rodents and humans (modified from Kumpun et al., 2011). The circles and arrows in red highlight the changes associated with 14-dehydroxylation, while the boxes and arrows in blue highlight the changes associated with side-chain cleavage.

Techniques: Modification

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: A representative flow-diagram for the large-scale extraction and purification of 20E from roots of Cyanotis sp.

Techniques: Extraction, Purification

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Drugability scores for ecdysteroids.

Techniques:

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: Clinical studies on humans using pure 20E.

Techniques: Activity Assay, Clinical Proteomics, Control

Journal: Biomedicines

Article Title: 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

doi: 10.3390/biomedicines9050492

Figure Lengend Snippet: SARS-CoV-2 infection will result in a strong impairment of the activity of angiotensin converting enzyme 2 (ACE2), hence a lack of angiotensin-(1-7) production and a disequilibrium between the harmful and protective arms of the renin–angiotensin system (RAS). Treatment with BIO101 (20E) is expected to activate Mas receptor and the protective arm of RAS, thus preventing inflammation and lung damage.

Techniques: Infection, Activity Assay

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